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Updated in [September 19th, 2023]
What does this course tell?
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Cell culture is the process by which cells are grown under controlled conditions generally outside their natural environment After the cells of interest have been isolated from living tissue they can subsequently be maintained under carefully controlled conditions These conditions vary for each cell type but generally consist of a suitable vessel with a substrate or medium that supplies the essential nutrients (amino acids carbohydrates vitamins minerals) growth factors hormones and gases (CO2 O2) and regulates the physio-chemical environment (pH buffer osmotic pressure temperature) Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture) The lifespan of most cells is genetically determined but some cell culturing cells have been [transformed] into immortal cells which will reproduce indefinitely if the optimal conditions are providedIn practice the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes especially animal cells in contrast with other types of culture that also grow cells such as plant tissue culture fungal culture and microbiological culture (of microbes) The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture Viral culture is also related with cells as hosts for the virusesMonoclonal antibodies (mAb or moAb) are antibodies that are made by identical immune cells that are all clones of a unique parent cell Monoclonal antibodies can have monovalent affinity in that they bind to the same epitope (the part of an antigen that is recognized by the antibody) In contrast polyclonal antibodies bind to multiple epitopes and are usually made by several different plasma cell (antibody secreting immune cell) lineages Bispecific monoclonal antibodies can also be engineered by increasing the therapeutic targets of one single monoclonal antibody to two epitopesGiven almost any substance it is possible to produce monoclonal antibodies that specifically bind to that substance; they can then serve to detect or purify that substance This has become an important tool in biochemistry molecular biology and medicine When used as medications non-proprietary drug names end in -mab (see "Nomenclature of monoclonal antibodies") and many immunotherapy specialists use the word mab anacronymicallyThese questions will give you basic idea for Examination Preparation and&or interview on Vectors Uses for Animal Cell Culture and Monoclonal AntibodiesPlease Note: These questions are only for practice and understanding level of knowledge only It is not necessary that these questions may or may not appear for examinations and&or interview questionsIn this practice test because of large amount of questions (around 57 questions) some of questions may have repeatedI had to put as 70% pass rate because there may also be wrong answers from my sideWe consider the value of this course from multiple aspects, and finally summarize it for you from three aspects: personal skills, career development, and further study:
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What skills and knowledge will you acquire during this course?
During this course on Cell Culture and Monoclonal Antibodies, the learner will acquire a range of skills and knowledge. They will learn about the process of cell culture, which involves growing cells under controlled conditions outside their natural environment. This includes understanding the different conditions required for various cell types, such as the necessary nutrients, growth factors, hormones, and gases. The learner will also learn about the different types of cell culture, including adherent or monolayer culture and suspension culture.
Additionally, the course will cover the historical development and methods of cell culture, which are closely related to tissue culture and organ culture. The learner will gain knowledge about viral culture and its relationship with cells as hosts for viruses.
Furthermore, the course will delve into the topic of monoclonal antibodies. The learner will understand that monoclonal antibodies are made by identical immune cells that are clones of a unique parent cell. They will learn about the differences between monoclonal antibodies and polyclonal antibodies, including their binding affinity and therapeutic targets. The learner will also gain an understanding of how monoclonal antibodies can be engineered to bind to specific substances, making them useful in detection and purification processes in biochemistry, molecular biology, and medicine.
Overall, this course will equip the learner with the skills and knowledge necessary to understand and apply cell culture techniques and the use of monoclonal antibodies in various scientific and medical contexts.
How does this course contribute to professional growth?
This course on Cell Culture and Monoclonal Antibodies contributes to professional growth by providing individuals with a comprehensive understanding of the principles and techniques involved in cell culture and the production of monoclonal antibodies.
By studying cell culture, individuals gain knowledge on how to grow and maintain cells under controlled conditions, which is essential in various fields such as biotechnology, pharmaceuticals, and medical research. This knowledge allows professionals to work with cells outside their natural environment, enabling them to conduct experiments, study cell behavior, and develop new therapies or treatments.
Additionally, learning about monoclonal antibodies equips individuals with the skills to produce antibodies that specifically bind to a particular substance. This ability is crucial in biochemistry, molecular biology, and medicine, as monoclonal antibodies can be used for detection, purification, and targeted therapy. Understanding the production and application of monoclonal antibodies enhances professionals' capabilities in research, diagnostics, and the development of therapeutic interventions.
Overall, this course expands professionals' knowledge and skills in cell culture and monoclonal antibody production, enabling them to contribute to advancements in various scientific and medical fields. It equips individuals with the expertise needed to conduct research, develop new treatments, and contribute to the overall progress of biotechnology and healthcare industries.
Is this course suitable for preparing further education?
This course on Cell Culture and Monoclonal Antibodies MCQ (Bio Tech) appears to be suitable for preparing for further education. The course covers topics such as cell culture techniques, the importance of controlled conditions, the role of nutrients and growth factors, and the use of monoclonal antibodies in biochemistry, molecular biology, and medicine. These are all fundamental concepts that are relevant to further studies in the field of biotechnology. However, it is important to note that the effectiveness of this course in preparing for further education may vary depending on the specific requirements and curriculum of the desired educational program.
Course Syllabus
Practice Tests